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Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves.

ABSTRACT: Bovine alphaherpesvirus 1 (BoHV-1) is a pathogen causing respiratory and reproductive clinical signs in cattle. Infected animals may develop rhinotracheitis, vulvovaginitis, balanoposthitis, and abortion. Viral latency is generally established in neuronal ganglia simultaneously to a decrease in both genes or genome expression and viral replication. Under stressful conditions, infection is reactivated leading to viral replication and the manifestation of clinical signs. In this study, we evaluated both viral reactivation and apoptosis in trigeminal ganglia cells as BoHV-1 progressed from the latent to the acute phase of infection after dexamethasone administration in experimentally infected calves. To test ganglia cell death as a consequence of BoHV-1 infection, we stained the BoHV-1 samples with TUNEL after the viral shedding by the calves. RT-qPCR of apoptotic genes was also performed, showing the upregulation of the caspase 8 gene in the trigeminal ganglia from cattle experimentally infected with BoHV-1. These results showed the occurrence of apoptosis in ganglion cells of calves infected by BoHV-1.

SUBMITTER: Ferreira HCC

PROVIDER: S-EPMC8578482 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves.

Ferreira Hanna Carolina Campos HCC de Araújo Elaine Nery EN Rosado Nívia Carolina Lopes NCL Fietto Juliana Lopes Rangel JLR Santos Marcus Rebouças MR Gomes Lidiany Lopes LL Silva Laura Morais Nascimento LMN Bressan Gustavo Costa GC Martins Gustavo Ferreira GF Sreevatsan Srinand S Silva-Júnior Abelardo A

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20210806 4

Bovine alphaherpesvirus 1 (BoHV-1) is a pathogen causing respiratory and reproductive clinical signs in cattle. Infected animals may develop rhinotracheitis, vulvovaginitis, balanoposthitis, and abortion. Viral latency is generally established in neuronal ganglia simultaneously to a decrease in both genes or genome expression and viral replication. Under stressful conditions, infection is reactivated leading to viral replication and the manifestation of clinical signs. In this study, we evaluate ...[more]

PMID: 34355356

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Dataset Information

Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves.

Publications

Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves.

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