DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts.

ABSTRACT: Accumulating evidence suggests that developmentally regulated GTP-binding protein 2 (DRG2), an evolutionarily conserved GTP-binding protein, plays an important role in regulating cell growth, inflammation, and mitochondria dynamics. However, the effect of DRG2 in aging remains unclear. In this study, we found that endogenous DRG2 protein expression is upregulated in oxidative stress-induced premature senescence models and tissues of aged mice. Ectopic expression of DRG2 significantly promoted senescence-associated β-galactosidase (SA-β-gal) activity and inhibited cell growth, concomitant with increase in levels of acetyl (ac)-p53 (Lys382), ac-nuclear factor-kB (NF-κB) p65 (Lys310), p21 ^Waf1/Cip1 , and p16 ^Ink4a and a decrease in cycl

SUBMITTER: Li BS 

PROVIDER: S-EPMC8580627 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature