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NAD-seq for profiling the NAD⁺ capped transcriptome of Arabidopsis thaliana.

ABSTRACT: Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD⁺) cap. NAD-seq identifies transcriptome-wide NAD⁺ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD⁺ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD⁺ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation. For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).

SUBMITTER: Yu X

PROVIDER: S-EPMC8593656 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana.

Yu Xiang X Vandivier Lee E LE Gregory Brian D BD

STAR protocols 20211111 4

Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library prepara ...[more]

PMID: 34816126

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