Production of 11α-hydroxysteroids from sterols in a single fermentation step by Mycolicibacterium smegmatis.

ABSTRACT: 11α-hydroxylated steroid synthons are one of the most important commercially pharmaceutical intermediates used for the production of contraceptive drugs and glucocorticoids. These compounds are currently produced by biotransformation using fungal strains in two sequential fermentation steps. In this work, we have developed by a rational design new recombinant bacteria able to produce 11α-hydroxylated synthons in a single fermentation step using cholesterol (CHO) or phytosterols (PHYTO) as feedstock. We have designed a synthetic operon expressing the 11α-hydroxylating enzymes from the fungus Rhizopus oryzae that was cloned into engineered mutant strains of Mycolicibacterium smegmatis that were previously created to produce 4-androstene-3,17-dione (AD), 1,4-androstadiene-3,17-dione (ADD) fro

SUBMITTER: Felpeto-Santero C 

PROVIDER: S-EPMC8601193 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature