Evaluating whole-genome sequencing quality metrics for enteric pathogen outbreaks.

ABSTRACT:

Background

Whole genome sequencing (WGS) has gained increasing importance in responses to enteric bacterial outbreaks. Common analysis procedures for WGS, single nucleotide polymorphisms (SNPs) and genome assembly, are highly dependent upon WGS data quality.

Methods

Raw, unprocessed WGS reads from Escherichia coli, Salmonella enterica, and Shigella sonnei outbreak clusters were characterized for four quality metrics: PHRED score, read length, library insert size, and ambiguous nucleotide composition. PHRED scores were strongly correlated with improved SNPs analysis results in E. coli and S. enterica clusters.

Results

Assembly quality showed only moderate correlations with PHRED scores and library insert size, and then only for Sal

SUBMITTER: Wagner DD 

PROVIDER: S-EPMC8627651 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature