In Vitro Oxidative Crosslinking of Recombinant Barnacle Cyprid Cement Gland Proteins.

ABSTRACT: Barnacle adhesion is a focus for fouling-control technologies as well as the development of bioinspired adhesives, although the mechanisms remain very poorly understood. The barnacle cypris larva is responsible for surface colonisation. Cyprids release cement from paired glands that contain proteins, carbohydrates and lipids, although further compositional details are scant. Several genes coding for cement gland-specific proteins were identified, but only one of these showed database homology. This was a lysyl oxidase-like protein (lcp_LOX). LOX-like enzymes have been previously identified in the proteome of adult barnacle cement secretory tissue. We attempted to produce recombinant LOX in E. coli, in order to identify its role in cyprid cement polymerisation. We also produced two other ce

SUBMITTER: Cleverley R 

PROVIDER: S-EPMC8639568 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature