Dataset Information

The Etiology of Pneumonia From Analysis of Lung Aspirate and Pleural Fluid Samples: Findings From the Pneumonia Etiology Research for Child Health (PERCH) Study.

ABSTRACT:

Background

An improved understanding of childhood pneumonia etiology is required to inform prevention and treatment strategies. Lung aspiration is the gold standard specimen for pneumonia diagnostics. We report findings from analyses of lung and pleural aspirates collected in the Pneumonia Etiology Research for Child Health (PERCH) study.

Methods

The PERCH study enrolled children aged 1-59 months hospitalized with World Health Organization-defined severe or very severe pneumonia in 7 countries in Africa and Asia. Percutaneous transthoracic lung aspiration (LA) and pleural fluid (PF) aspiration was performed on a sample of pneumonia cases with radiological consolidation and/or PF in 4 countries. Venous blood and nasopharyngeal/oropharyngeal swabs were collected from all cases. Multiplex quantitative polymerase chain reaction (PCR) and routine microbiologic culture were applied to clinical specimens.

Results

Of 44 LAs performed within 3 days of admission on 622 eligible cases, 13 (30%) had a pathogen identified by either culture (5/44) or by PCR (11/29). A pathogen was identified in 12/14 (86%) PF specimens tested by either culture (9/14) or PCR (9/11). Bacterial pathogens were identified more frequently than viruses. All but 1 of the cases with a virus identified were coinfected with bacterial pathogens. Streptococcus pneumoniae (9/44 [20%]) and Staphylococcus aureus (7/14 [50%]) were the predominant pathogens identified in LA and PF, respectively.

Conclusions

Bacterial pathogens predominated in this selected subgroup of PERCH participants drawn from those with radiological consolidation or PF, with S. pneumoniae and S. aureus the leading pathogens identified.

SUBMITTER: Ebruke BE

PROVIDER: S-EPMC8662778 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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The Etiology of Pneumonia From Analysis of Lung Aspirate and Pleural Fluid Samples: Findings From the Pneumonia Etiology Research for Child Health (PERCH) Study.

Ebruke Bernard E BE Deloria Knoll Maria M Haddix Meredith M Zaman Syed M A SMA Prosperi Christine C Feikin Daniel R DR Hammitt Laura L LL Levine Orin S OS O'Brien Katherine L KL Murdoch David R DR Brooks W Abdullah WA Scott J Anthony G JAG Kotloff Karen L KL Madhi Shabir A SA Thea Donald M DM Baillie Vicky L VL Chisti Mohammod Jobayer MJ Dione Michel M Driscoll Amanda J AJ Fancourt Nicholas N Karron Ruth A RA Le Tham T TT Mohamed Shebe S Moore David P DP Morpeth Susan C SC Mwaba John J Mwansa James J Bin Shahid Abu Sadat Mohammad Sayeem ASMS Sow Samba O SO Tapia Milagritos D MD Antonio Martin M Howie Stephen R C SRC

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 20211201 11

<h4>Background</h4>An improved understanding of childhood pneumonia etiology is required to inform prevention and treatment strategies. Lung aspiration is the gold standard specimen for pneumonia diagnostics. We report findings from analyses of lung and pleural aspirates collected in the Pneumonia Etiology Research for Child Health (PERCH) study.<h4>Methods</h4>The PERCH study enrolled children aged 1-59 months hospitalized with World Health Organization-defined severe or very severe pneumonia i ...[more]

PMID: 32710751

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Dataset Information

The Etiology of Pneumonia From Analysis of Lung Aspirate and Pleural Fluid Samples: Findings From the Pneumonia Etiology Research for Child Health (PERCH) Study.

Background

Methods

Results

Conclusions

Publications

The Etiology of Pneumonia From Analysis of Lung Aspirate and Pleural Fluid Samples: Findings From the Pneumonia Etiology Research for Child Health (PERCH) Study.

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