Dataset Information

Sterculia tragacantha Lindl Leaf Extract Ameliorates STZ-Induced Diabetes, Oxidative Stress, Inflammation and Neuronal Impairment.

ABSTRACT:

Background

Sterculia tragacantha is a medicinal plant commonly used in the western part of Nigeria, for managing diabetes mellitus. However, there is a dearth of scientific information on the antidiabetic and neuroprotective properties of the plant.

Methods

The in silico, in vitro and in vivo models were used to evaluate the antioxidants, antidiabetic, anti-inflammatory and neuroprotective potential of aqueous extract of Sterculia tragacantha leaf (AESTL) in streptozotocin (STZ)-induced diabetic rats. Thirty (30) male albino rats (155.34±6.33 g) were intraperitoneal injected with 40 mg/kg of freshly prepared streptozotocin and were divided into 5 groups (A-E) of 6 animals each. Groups A-D were treated with 0, 150 and 300 mg/kg of AESTL, and 200 mg/kg body weight of metformin respectively, while group E serve as the normal control.

Results

The results of in vitro analysis revealed dose-dependent antioxidant activities; ABTS (IC₅₀ = 63.03±2.57 μg/mL), DPPH (117.49±2.35 μg/mL), FRAP (15.19±0.98 mmol/100g), TAC (43.38±0.96 mg/100g), hypoglycaemic effect; α-amylase (IC₅₀ = 77.21±4.35 μg/mL) and α-glucosidase (IC₅₀ = 443.25±12.35), and anti-cholinesterase; AChE (IC₅₀ = 113.07±3.42 μg/mL) and BChE (IC₅₀ = 87.50±4.32 μg/mL) activities of AESTL. In vivo study revealed dose-dependent hypoglycemic effect and body weight improvement in rats treated with the AESTL. In addition, AESTL improved the antioxidant status and attenuated STZ-induced dysregulations of Na⁺-K⁺-ATPase, cholinesterases and neurotransmitters in the brain tissue of experimental rats. The results also demonstrated that AESTL could regulate anti-inflammatory response via inhibition of COX-2/NO signaling axis in the brain of diabetic rats. Molecular docking analysis revealed that epicatechin and procyanidin B2, the bioactive compounds from AESTL, docked well to the binding cavities of acetylcholinesterase, butyrylcholinesterase, α-amylase and α-glucosidase with binding affinities ranges between -8.0 and -11.4 kcal/mol, suggesting that these compounds are the bioactive component that could be responsible for the antidiabetic and neuroprotective activities of AESTL.

Conclusion

The results of the present study strongly suggested that the AESTL extract could be very useful for halting diabetes progression and its associated neuroinflammation complications.

SUBMITTER: Onikanni AS

PROVIDER: S-EPMC8668250 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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<i>Sterculia tragacantha</i> Lindl Leaf Extract Ameliorates STZ-Induced Diabetes, Oxidative Stress, Inflammation and Neuronal Impairment.

Onikanni Amos Sunday AS Lawal Bashir B Olusola Augustine O AO Olugbodi Janet O JO Sani Saidu S Ajiboye Basiru Olaitan BO Ilesanmi Omotayo B OB Alqarni Mohammed M Mostafa-Hedeab Gomaa G Obaidullah Ahmad J AJ Batiha Gaber El-Saber GE Wu Alexander T H ATH

Journal of inflammation research 20211209

<h4>Background</h4><i>Sterculia tragacantha</i> is a medicinal plant commonly used in the western part of Nigeria, for managing diabetes mellitus. However, there is a dearth of scientific information on the antidiabetic and neuroprotective properties of the plant.<h4>Methods</h4>The in silico, in vitro and in vivo models were used to evaluate the antioxidants, antidiabetic, anti-inflammatory and neuroprotective potential of aqueous extract of <i>Sterculia tragacantha</i> leaf (AESTL) in streptoz ...[more]

PMID: 34916823

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