Combined Metallomics/Transcriptomics Profiling Reveals a Major Role for Metals in Wound Repair.

ABSTRACT: Endogenous metals are required for all life, orchestrating the action of diverse cellular processes that are crucial for tissue function. The dynamic wound healing response is underpinned by a plethora of such cellular behaviours, occurring in a time-dependent manner. However, the importance of endogenous metals for cutaneous repair remains largely unexplored. Here we combine ICP-MS with tissue-level RNA-sequencing to reveal profound changes in a number of metals, and corresponding metal-regulated genes, across temporal healing in mice. Wound calcium, magnesium, iron, copper and manganese are elevated at 7 days post-wounding, while magnesium, iron, aluminium, manganese and cobalt increase at 14 days post-wounding. At the level of transcription, wound-induced pathways are independently high

SUBMITTER: Wilkinson HN 

PROVIDER: S-EPMC8669724 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature