Dataset Information

In vivo optogenetic stimulation of the primate retina activates the visual cortex after long-term transduction.

ABSTRACT: Over the last 15 years, optogenetics has changed fundamental research in neuroscience and is now reaching toward therapeutic applications. Vision restoration strategies using optogenetics are now at the forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading to blindness raise important concerns on the long-term functional expression of optogenes and the efficient signal transmission to higher visual centers. Here, we demonstrate in non-human primates continued expression and functionality at the retina level ∼20 months after delivery of our construct. We also performed in vivo recordings of visually evoked potentials in the primary visual cortex of anesthetized animals. Using synaptic blockers, we isolated the in vivo cortical activation resulting from the direct optogenetic stimulation of primate retina. In conclusion, our work indicates long-term transgene expression and transmission of the signal generated in the macaque retina to the visual cortex, two important features for future clinical applications.

SUBMITTER: Chaffiol A

PROVIDER: S-EPMC8671818 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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<i>In vivo</i> optogenetic stimulation of the primate retina activates the visual cortex after long-term transduction.

Chaffiol Antoine A Provansal Matthieu M Joffrois Corentin C Blaize Kévin K Labernede Guillaume G Goulet Ruben R Burban Emma E Brazhnikova Elena E Duebel Jens J Pouget Pierre P Sahel José Alain JA Picaud Serge S Arcizet Fabrice F Gauvain Gregory G

Molecular therapy. Methods & clinical development 20211122

Over the last 15 years, optogenetics has changed fundamental research in neuroscience and is now reaching toward therapeutic applications. Vision restoration strategies using optogenetics are now at the forefront of these new clinical opportunities. But applications to human patients suffering from retinal diseases leading to blindness raise important concerns on the long-term functional expression of optogenes and the efficient signal transmission to higher visual centers. Here, we demonstrate ...[more]

PMID: 34977267

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Dataset Information

In vivo optogenetic stimulation of the primate retina activates the visual cortex after long-term transduction.

Publications

<i>In vivo</i> optogenetic stimulation of the primate retina activates the visual cortex after long-term transduction.

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