Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOB^T and Geobacter sulfurreducens PCA^T.

ABSTRACT: We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOB^T (SF) and Geobacter sulfurreducens PCA^T (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF c

SUBMITTER: Mollaei M 

PROVIDER: S-EPMC8691401 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature