Enhancing the methanol tolerance of Candida antarctica lipase B by saturation mutagenesis for biodiesel preparation.

ABSTRACT: Methanol tolerance of lipase is one of the important factors affecting its esterification ability in biodiesel preparation. By B factor indicated prediction of Candida antarctica lipase B (CalB) surface amino acids, eight sites (Val¹³⁹, Ala¹⁴⁶, Leu¹⁴⁷, Pro²¹⁸, Val²⁸⁶, Ala²⁸⁷, Val³⁰⁶, and Gly³⁰⁷) with high B value indicating more flexibility were chosen to perform saturation mutagenesis. High-methanol-tolerant variants, CalB-P218W and -V306N, created larger haloes on emulsified tributyrin solid plate including 15% (v/v) methanol and showed 19% and 31% higher activity over wild-type CalB (CalB-WT), respectively. By modeling, a newly formed hydrogen bond in CalB-V306N and hydrophobic f

SUBMITTER: Tan Z 

PROVIDER: S-EPMC8695645 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature