ORAI1 Limits SARS-CoV-2 Infection by Regulating Tonic Type I IFN Signaling.

ABSTRACT: ORAI1 and stromal interaction molecule 1 (STIM1) are the critical mediators of store-operated Ca²⁺ entry by acting as the pore subunit and an endoplasmic reticulum-resident signaling molecule, respectively. In addition to Ca²⁺ signaling, STIM1 is also involved in regulation of the type I IFN (IFN-I) response. To examine their potential role in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, we generated ORAI1 and STIM1 knockout human HEK293-angiotensin-converting enzyme 2 cells and checked their responses. STIM1 knockout cells showed strong resistance to SARS-CoV-2 infection as a result of enhanced IFN-I response. On the contrary, ORAI1 deletion induced high susceptibility to SARS-CoV-2 infection. Mechanistically,

SUBMITTER: Wu B 

PROVIDER: S-EPMC8702473 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature