Extrachromosomal DNA in HPV-Mediated Oropharyngeal Cancer Drives Diverse Oncogene Transcription.

ABSTRACT:

Purpose

Human papillomavirus (HPV) plays a major role in oncogenesis and circular extrachromosomal DNA (ecDNA) is found in many cancers. However, the relationship between HPV and circular ecDNA in human cancer is not understood.

Experimental design

Forty-four primary tumor tissue samples were obtained from a cohort of patients with HPV-positive oropharynx squamous cell carcinoma (OPSCC). Twenty-eight additional HPV oropharyngeal cancer (HPVOPC) tumors from The Cancer Genome Atlas (TCGA) project were analyzed as a separate validation cohort. Genomic, transcriptomic, proteomic, computational, and functional analyses of HPVOPC were applied to these datasets.

Results

Our analysis revealed circular, oncogenic DNA in nearly all HPVOPC, with circular human and human-viral hy

SUBMITTER: Pang J 

PROVIDER: S-EPMC8710294 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature