Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scaling.

ABSTRACT: The function of cellular structures at the mesoscale is dependent on their geometry and proportionality to cell size. The mitotic spindle is a good example why length and shape of intracellular organelles matter. Spindle length determines the distance over which chromosomes will segregate, and spindle shape ensures bipolarity. While we still lack a systematic and quantitative understanding of subcellular morphology, new imaging techniques and volumetric data analysis promise novel insights into scaling relations across different species. Here, we introduce Spindle3D, an open-source plug-in that allows for the quantitative, consistent, and automated analysis of 3D fluorescent data of spindles and chromatin. We systematically analyze different mammalian cell types, including somatic cells, s

SUBMITTER: Kletter T 

PROVIDER: S-EPMC8719715 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature