Large-Scale Proteomic Assessment of Urinary Extracellular Vesicles Highlights Their Reliability in Reflecting Protein Changes in the Kidney.

ABSTRACT:

Background

Urinary extracellular vesicles (uEVs) are secreted into urine by cells from the kidneys and urinary tract. Although changes in uEV proteins are used for quantitative assessment of protein levels in the kidney or biomarker discovery, whether they faithfully reflect (patho)physiologic changes in the kidney is a matter of debate.

Methods

Mass spectrometry was used to compare in an unbiased manner the correlations between protein levels in uEVs and kidney tissue from the same animal. Studies were performed on rats fed a normal or high K⁺ diet.

Results

Absolute quantification determined a positive correlation (Pearson R=0.46 or 0.45, control or high K⁺ respectively, P<0.0001) between the approximately 1000 proteins identified

SUBMITTER: Wu Q 

PROVIDER: S-EPMC8729841 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature