High-Throughput Culture Method of Induced Pluripotent Stem Cell-Derived Alveolar Epithelial Cells.

ABSTRACT: Lung regeneration is dependent on the availability of progenitor lung cells. Large numbers of self-renewing, patient-specific induced pluripotent stem cell-derived alveolar epithelial cells (iPSC-AECs) are needed to adequately recellularize whole-organ constructs. Prior methods to generate functional iPSC-AECs are not feasible for large-scale cell production. We present a novel protocol to produce iPSC-AECs, which is scalable for whole-organ regeneration. Differentiation of iPSCs was performed with genetically modified iPSCs with fluorescent reporters, which underwent differentiation in a stepwise protocol mimicking lung development. Cells were purified, sorted, and embedded in a liquid Matrigel precursor either to form adherent droplets or to form cell-laden Matrigel spheroids, which were

SUBMITTER: Becerra D 

PROVIDER: S-EPMC8742269 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature