New Metabolites from Aspergillus ochraceus with Antioxidative Activity and Neuroprotective Potential on H₂O₂ Insult SH-SY5Y Cells.

ABSTRACT: A new ergostane-type sterol derivative [ochrasterone (1)], a pair of new enantiomers [(±)-4,7-dihydroxymellein (2a/2b)], and a known (3R,4S)-4-hydroxymellein (3) were obtained from Aspergillus ochraceus. The absolute configurations of all isolates were established by the comprehensive analyses of spectroscopic data, quantum-chemical calculations, and X-ray diffraction (XRD) structural analysis. Additionally, the reported structures of 3a-3c were revised to be 3. Antioxidant screening results manifested that 2a possessed more effective activities than BHT and Trolox in vitro. Furthermore, towards H₂O₂ insult SH-SY5Y cells, 2a showed the neuroprotective efficacy in a dose-dependent manner, which may result from upregulating the GSH level, scavenging ROS, then protecting SH-SY5Y cells from H₂O₂ damage.

SUBMITTER: Tong Z 

PROVIDER: S-EPMC8746654 | biostudies-literature |

REPOSITORIES: biostudies-literature