ADAMTS5 is required for normal trabeculated bone development in the mandibular condyle.

ABSTRACT:

Objective

Determine the role of the extracellular matrix protease ADAMTS5 in development of the trabeculated bone of the mandibular condyle.

Methods

The mandibular condyles of wild type and mice deficient in the protease ADAMTS5 were examined for histopathology with Safranin O staining. Microcomputed tomography was performed to analyze the developing bone of the mandibular condyle. RNAscope and immunohistochemistry were utilized to investigate cell type and extracellular matrix expression.

Results

Mice deficient in Adamts5, (Adamts5^tm1Dgen/J) exhibit an increase in trabecular separation (n = 37 wild type; n = 27: P < 0.0001) and reduction of trabecular thickness P = 0.0116 and bone volume fraction P = 0.0869 in the mandibular condylar head compared to wild

SUBMITTER: Rogers-DeCotes AW 

PROVIDER: S-EPMC8759092 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature