Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35⁺ B cell generation.

ABSTRACT:

Background

IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition. However, whether the gut microbiota regulates these IL-35⁺ cells remains elusive. We herein investigated the regulatory effects of the gut microbiota on IL-35⁺ cells by using genetically modified mouse models of obesity.

Results

We first found that gut Reg4 promoted resistance to high-fat diet-induced obesity. Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35⁺ B cells through 3-idoleacetic acid (IAA) in the presence of LPS. HuREG4<

SUBMITTER: Su X 

PROVIDER: S-EPMC8785567 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature