Red cell proteasome modulation by storage, redox metabolism and transfusion.

ABSTRACT:

Background

Proteasomes are proteolytic complexes with prominent roles in the control of protein homeostasis and cellular viability. However, little is known about the effects of storage and glucose-6-phosphate dehydrogenase deficiency (G6PD^-) on the activity and topology of red blood cell (RBC) proteasomes.

Materials and methods

We investigated the concentration (by GeLC-MS proteomics analysis and immunoblotting), activity (by using peptide substrates and proteasome inhibitors), and subcellular/extracellular distribution (following cell fractionation and isolation of extracellular vesicles, respectively) of RBC proteasomes in fresh blood and RBCs from control and G6PD^- donors following storage in leukoreduced units. RBC proteasome activity was also test

SUBMITTER: Tzounakas VL 

PROVIDER: S-EPMC8796840 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature