Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.

ABSTRACT:

Introduction

This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.

Methods

For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.

Results

The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks ass

SUBMITTER: Bielajew BJ 

PROVIDER: S-EPMC8804780 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature