Regulation of circadian rhythm and sleep by miR-375-timeless interaction in Drosophila.

ABSTRACT: MicroRNAs are important coordinators of circadian regulation that mediate the fine-tuning of gene expression. Although many studies have shown the effects of individual miRNAs on the circadian clock, the global functional miRNA-mRNA interaction network involved in the circadian system remains poorly understood. Here, we used CLEAR (Covalent Ligation of Endogenous Argonaute-bound RNAs)-CLIP (Cross-Linking and Immuno-Precipitation) to explore the regulatory functions of miRNAs in the circadian system by comparing the miRNA-mRNA interactions between Drosophila wild-type strain W¹¹¹⁸ and a mutant of the key circadian transcriptional regulator Clock (Clk^jrk ). This experimental approach unambiguously identified tens of thousands of miRNA-mRNA interactions in both the head

SUBMITTER: Xia X 

PROVIDER: S-EPMC8805441 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature