Accessible Region Conformation Capture (ARC-C) gives high-resolution insights into genome architecture and regulation.

ABSTRACT: Nuclear organization and chromatin interactions are important for genome function, yet determining chromatin connections at high resolution remains a major challenge. To address this, we developed Accessible Region Conformation Capture (ARC-C), which profiles interactions between regulatory elements genome-wide without a capture step. Applied to Caenorhabditis elegans, ARC-C identifies approximately 15,000 significant interactions between regulatory elements at 500-bp resolution. Of 105 TFs or chromatin regulators tested, we find that the binding sites of 60 are enriched for interacting with each other, making them candidates for mediating interactions. These include cohesin and condensin II. Applying ARC-C to a mutant of transcription factor BLMP-1 detected changes in interactions

SUBMITTER: Huang N 

PROVIDER: S-EPMC8805715 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature