Sensitivity and Isoform Specificity of ¹⁸F-Fluorofuranylnorprogesterone for Measuring Progesterone Receptor Protein Response to Estradiol Challenge in Breast Cancer.

ABSTRACT: The purpose of this study was to evaluate the ability of 21-¹⁸F-fluoro-16α,17α-[(R)-(1'-α-furylmethylidene)dioxy]-19-norpregn-4-ene-3,20-dione (¹⁸F-FFNP) to measure alterations in progesterone receptor (PR) protein level and isoform expression in response to estradiol challenge. Methods: T47D human breast cancer cells and female mice-bearing T47D tumor xenografts were treated with 17β-estradiol (E2) to increase PR expression. ¹⁸F-FFNP uptake was measured using cell uptake and tissue biodistribution assays. MDA-MB-231 breast cancer clonal cell lines were generated that express the A or B isoforms of human PR. PR protein levels, transcriptional function, and subcellular localization were determined. In vitro ¹⁸F-FFNP binding was measured via

SUBMITTER: Salem K 

PROVIDER: S-EPMC8833853 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature