Autotrophic lactate production from H₂ + CO₂ using recombinant and fluorescent FAST-tagged Acetobacterium woodii strains.

ABSTRACT: Lactate has various uses as industrial platform chemical, poly-lactic acid precursor or feedstock for anaerobic co-cultivations. The aim of this study was to construct and characterise Acetobacterium woodii strains capable of autotrophic lactate production. Therefore, the lctBCD genes, encoding the native Lct dehydrogenase complex, responsible for lactate consumption, were knocked out. Subsequently, a gene encoding a D-lactate dehydrogenase (LDHD) originating from Leuconostoc mesenteroides was expressed in A. woodii, either under the control of the anhydrotetracycline-inducible promoter P_tet or under the lactose-inducible promoter P_bgaL. Moreover, LDHD was N-terminally fused to the oxygen-independent fluorescence-activating and absorption-shifting tag (FAST) and expre

SUBMITTER: Mook A 

PROVIDER: S-EPMC8882112 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature