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A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development.

ABSTRACT: Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug discovery tool to accelerate the drug development for a wide range of physiological processes, such as cancer, neurological, cardiopulmonary, metabolic, and infectious diseases including COVID-19.

SUBMITTER: Reyes-Alcaraz A

PROVIDER: S-EPMC8904512 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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A NanoBiT assay to monitor membrane proteins trafficking for drug discovery and drug development.

Reyes-Alcaraz Arfaxad A Lucero Garcia-Rojas Emilio Y EY Merlinsky Elizabeth A EA Seong Jae Young JY Bond Richard A RA McConnell Bradley K BK

Communications biology 20220308 1

Internalization of membrane proteins plays a key role in many physiological functions; however, highly sensitive and versatile technologies are lacking to study such processes in real-time living systems. Here we describe an assay based on bioluminescence able to quantify membrane receptor trafficking for a wide variety of internalization mechanisms such as GPCR internalization/recycling, antibody-mediated internalization, and SARS-CoV2 viral infection. This study represents an alternative drug ...[more]

PMID: 35260793

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