Unraveling the Proteomic Landscape of Intestinal Epithelial Cell-Derived Exosomes in Mice.

ABSTRACT:

Purpose

This study aimed to identify the biological functions of small intestine intestinal epithelial cell derived exosomes (IEC-Exos) and further distinguished the difference proteins in IEC-Exos between ileum and jejunum related to function of the digestive system and occurrence of several diseases.

Materials and methods

IECs of Male C57BL/6J mice were isolated. IEC-Exos were extracted from jejunum and ileum epithelial cell culture fluid by ultracentrifugation. In addition, isobaric tags for relative and absolute quantitation (iTRAQ) combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to detect IEC-Exo proteins and conduct biological information analysis.

Results

The results showed that compared with jejunum IEC-Exos from ileum IEC-Exo

SUBMITTER: Ding Z 

PROVIDER: S-EPMC8905357 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature