A Novel LHX6 Reporter Cell Line for Tracking Human iPSC-Derived Cortical Interneurons.

ABSTRACT: GABAergic interneurons control the neural circuitry and network activity in the brain. The dysfunction of cortical interneurons, especially those derived from the medial ganglionic eminence, contributes to neurological disease states. Pluripotent stem cell-derived interneurons provide a powerful tool for understanding the etiology of neuropsychiatric disorders, as well as having the potential to be used as medicine in cell therapy for neurological conditions such as epilepsy. Although large numbers of interneuron progenitors can be readily induced in vitro, the generation of defined interneuron subtypes remains inefficient. Using CRISPR/Cas9-assisted homologous recombination in hPSCs, we inserted the coding sequence of mEmerald and mCherry fluorescence protein, respectively, downstream tha

SUBMITTER: Cruz-Santos M 

PROVIDER: S-EPMC8909769 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature