Genome-Wide Transcriptional Response of Mycobacterium smegmatis MC²155 to G-Quadruplex Ligands BRACO-19 and TMPyP4.

ABSTRACT: G-quadruplexes (G4s) are non-canonical DNA structures that could be considered as potential therapeutic targets for antimicrobial compounds, also known as G4-stabilizing ligands. While some of these ligands are shown in vitro to have a stabilizing effect, the precise mechanism of antibacterial action has not been fully investigated. Here, we employed genome-wide RNA-sequencing to analyze the response of Mycobacterium smegmatis to inhibitory concentrations of BRACO-19 and TMPyP4 G4 ligands. The expression profile changed (FDR < 0.05, log₂FC > |1|) for 822 (515↑; 307↓) genes in M. smegmatis in response to BRACO-19 and for 680 (339↑; 341↓) genes in response to TMPyP4. However, the analysis revealed no significant ligand-induced changes in the expression levels

SUBMITTER: Shitikov E 

PROVIDER: S-EPMC8931766 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature