Characterization and reduction of non-endocrine cells accompanying islet-like endocrine cells differentiated from human iPSC.

ABSTRACT: The differentiation of pancreatic endocrine cells from human pluripotent stem cells has been thoroughly investigated for their application in cell therapy against diabetes. Although non-endocrine cells are inevitable contaminating by-products of the differentiation process, a comprehensive profile of such cells is lacking. Therefore, we characterized non-endocrine cells in iPSC-derived pancreatic islet cells (iPIC) using single-cell transcriptomic analysis. We found that non-endocrine cells consist of (1) heterogeneous proliferating cells, and (2) cells with not only pancreatic traits but also liver or intestinal traits marked by FGB or AGR2. Non-endocrine cells specifically expressed FGFR2, PLK1, and LDHB. We demonstrated that inhibition of pathways involving these genes selectively reduc

SUBMITTER: Hiyoshi H 

PROVIDER: S-EPMC8933508 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature