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In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.

ABSTRACT: In-vitro modeling of brain network disorders such as epilepsy remains a major challenge. A critical step is to develop an experimental approach that enables recapitulation of in-vivo-like three-dimensional functional complexity while allowing local modulation of the neuronal networks. Here, by promoting matrix-supported active cell reaggregation, we engineered multiregional cerebral tissues with intact 3D neuronal networks and functional interconnectivity characteristic of brain networks. Furthermore, using a multi-chambered tissue-culture chip, we show that our separated but interconnected cerebral tissues can mimic neuropathological signatures such as the propagation of epileptiform discharges.

SUBMITTER: Saberi A

PROVIDER: S-EPMC8943047 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.

Saberi Aref A Aldenkamp Albert P AP Kurniawan Nicholas A NA Bouten Carlijn V C CVC

Communications biology 20220323 1

In-vitro modeling of brain network disorders such as epilepsy remains a major challenge. A critical step is to develop an experimental approach that enables recapitulation of in-vivo-like three-dimensional functional complexity while allowing local modulation of the neuronal networks. Here, by promoting matrix-supported active cell reaggregation, we engineered multiregional cerebral tissues with intact 3D neuronal networks and functional interconnectivity characteristic of brain networks. Furthe ...[more]

PMID: 35322168

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Dataset Information

In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.

Publications

In-vitro engineered human cerebral tissues mimic pathological circuit disturbances in 3D.

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