Culture of patient-derived multicellular clusters in suspended hydrogel capsules for pre-clinical personalized drug screening.

ABSTRACT: A personalized medication regimen provides precise treatment for an individual and can be guided by pre-clinical drug screening. The economical and high-efficiency simulation of the liver tumor microenvironment (TME) in a drug-screening model has high value yet challenging to accomplish. Herein, we propose a simulation of the liver TME with suspended alginate-gelatin hydrogel capsules encapsulating patient-derived liver tumor multicellular clusters, and the culture of patient-derived tumor organoids(PDTOs) for personalized pre-clinical drug screening. The hydrogel capsule offers a 3D matrix environment with mechanical and biological properties similar to those of the liver in vivo. As a result, 18 of the 28 patient-derived multicellular clusters were successfully cultured as PDTOs.

SUBMITTER: Dong H 

PROVIDER: S-EPMC8961426 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature