Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications.

ABSTRACT: The distribution of photo-crosslinkable moieties onto a protein backbone can affect a biomaterial's crosslinking behavior, and therefore also its mechanical and biological properties. A profound insight in this respect is essential for biomaterials exploited in tissue engineering and regenerative medicine. In the present work, photo-crosslinkable moieties have been introduced on the primary amine groups of: (i) a recombinant collagen peptide (RCPhC1) with a known amino acid (AA) sequence, and (ii) bovine skin collagen (COL BS) with an unknown AA sequence. The degree of substitution (DS) was quantified with two conventional techniques: an ortho-phthalic dialdehyde (OPA) assay and ¹H NMR spectroscopy. However, neither of both provides information on the exact type and locat

SUBMITTER: Pien N 

PROVIDER: S-EPMC8965084 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature