Microbiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods.

ABSTRACT:

Background

Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers.

Aim

To analyze the microbiome of IDFU in association with clinical outcomes.

Methods

Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes.

Results

A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 5

SUBMITTER: Mudrik-Zohar H 

PROVIDER: S-EPMC8987016 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature