Promiscuity mapping of the S100 protein family using a high-throughput holdup assay.

ABSTRACT: S100 proteins are small, typically homodimeric, vertebrate-specific EF-hand proteins that establish Ca²⁺-dependent protein-protein interactions in the intra- and extracellular environment and are overexpressed in various pathologies. There are about 20 distinct human S100 proteins with numerous potential partner proteins. Here, we used a quantitative holdup assay to measure affinity profiles of most members of the S100 protein family against a library of chemically synthetized foldamers. The profiles allowed us to quantitatively map the binding promiscuity of each member towards the foldamer library. Since the library was designed to systematically contain most binary natural amino acid side chain combinations, the data also provide insight into the promiscuity of each S100 prot

SUBMITTER: Simon MA 

PROVIDER: S-EPMC8991199 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature