Oscillatory calcium release and sustained store-operated oscillatory calcium signaling prevents differentiation of human oligodendrocyte progenitor cells.

ABSTRACT: Endogenous remyelination in demyelinating diseases such as multiple sclerosis is contingent upon the successful differentiation of oligodendrocyte progenitor cells (OPCs). Signaling via the Gα_q-coupled muscarinic receptor (M_1/3R) inhibits human OPC differentiation and impairs endogenous remyelination in experimental models. We hypothesized that calcium release following Gα_q-coupled receptor (G_qR) activation directly regulates human OPC (hOPC) cell fate. In this study, we show that specific G_qR agonists activating muscarinic and metabotropic glutamate receptors induce characteristic oscillatory calcium release in hOPCs and that these agonists similarly block hOPC maturation in vitro. Both agonists induce calcium release from endoplasmic

SUBMITTER: Seidman RA 

PROVIDER: S-EPMC9007940 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature