Antigen identification and high-throughput interaction mapping by reprogramming viral entry.

ABSTRACT: Deciphering immune recognition is critical for understanding a broad range of diseases and for the development of effective vaccines and immunotherapies. Efforts to do so are limited by a lack of technologies capable of simultaneously capturing the complexity of adaptive immunoreceptor repertoires and the landscape of potential antigens. To address this, we present receptor-antigen pairing by targeted retroviruses, which combines viral pseudotyping and molecular engineering approaches to enable one-pot library-on-library interaction screens by displaying antigens on the surface of lentiviruses and encoding their identity in the viral genome. Antigen-specific viral infection of cell lines expressing human T or B cell receptors allows readout of both antigen and receptor identities via singl

SUBMITTER: Dobson CS 

PROVIDER: S-EPMC9012700 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature