MicroRNA profile of circulating CD4⁺ T cells in aged patients with atherosclerosis obliterans.

ABSTRACT:

Background

To evaluate the specificity of the expression patterns of microRNAs (miRNAs) in circulating CD4⁺ T cells in aged patients with atherosclerosis obliterans (ASO).

Methods

A comprehensive miRNA expression study was conducted using a miRNA microarray of CD4⁺ T cells isolated from peripheral blood mononuclear cells (PBMCs) of 33 patients with ASO and 24 healthy donors. A t test was used for statistical analysis, and the average linkage method was used for hierarchical clustering. The results were validated by qRT-PCR. Putative targeted pathways associated with validated miRNAs were predicted with the online software DIANA miRPath.

Results

We identified 44 miRNAs based on a cutoff value of a 1.3-fold change in expression between the two grou

SUBMITTER: Wang S 

PROVIDER: S-EPMC9013077 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature