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Sequencing Ultrarare Targets with Compound Nucleic Acid Cytometry.

ABSTRACT: Targeted sequencing enables sensitive and cost-effective analysis by focusing resources on molecules of interest. Existing methods, however, are limited in enrichment power and target capture length. Here, we present a novel method that uses compound nucleic acid cytometry to achieve million-fold enrichments of molecules >10 kbp in length using minimal prior target information. We demonstrate the approach by sequencing HIV proviruses in infected individuals. Our method is useful for rare target sequencing in research and clinical applications, including for identifying cancer-associated mutations or sequencing viruses infecting cells.

SUBMITTER: Sun C

PROVIDER: S-EPMC9022902 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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Sequencing Ultrarare Targets with Compound Nucleic Acid Cytometry.

Sun Chen C Chang Kai-Chun KC Abate Adam R AR

Analytical chemistry 20210510 20

Targeted sequencing enables sensitive and cost-effective analysis by focusing resources on molecules of interest. Existing methods, however, are limited in enrichment power and target capture length. Here, we present a novel method that uses compound nucleic acid cytometry to achieve million-fold enrichments of molecules >10 kbp in length using minimal prior target information. We demonstrate the approach by sequencing HIV proviruses in infected individuals. Our method is useful for rare target ...[more]

PMID: 33971091

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