Absolute Proteome Quantification in the Gas-Fermenting Acetogen Clostridium autoethanogenum.

ABSTRACT: Microbes that can recycle one-carbon (C₁) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO₂ and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H₂, or CO+CO₂+H₂). We detect the prioritization of proteome allocation for C₁ fixation and the significant expression of proteins involved in the production of acetate and ethanol as well as proteins with

SUBMITTER: Valgepea K 

PROVIDER: S-EPMC9040625 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature