Dataset Information

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay.

ABSTRACT: Herein, we synthesized high-performance SiO₂-core quantum dot (QD)-shell nanocomposites (SiO₂@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO₂ core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO₂@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels for the detection of bacteria when used with the lateral flow immunoassay (LFA) technique. An SiO₂@PEI-QD-based LFA strip was successfully applied to rapidly detect Salmonella typhimurium in milk samples with a low limit of 5 × 10² cells per mL.

SUBMITTER: Zhang B

PROVIDER: S-EPMC9048750 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay.

Zhang Bo B Yang Xingsheng X Liu Xiaoxian X Li Juan J Wang Chongwen C Wang Shengqi S

RSC advances 20200114 5

Herein, we synthesized high-performance SiO2-core quantum dot (QD)-shell nanocomposites (SiO2@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO2 core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO2@PEI-QDs showed excellent stability and high luminescence, and serv ...[more]

PMID: 35496136

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Project description:Brucellosis is a highly contagious zoonosis chronic infectious disease with a strong latent capability to endanger human health and economic development via direct or indirect ways. However, the existing methods for brucellosis diagnosis are time-consuming and expensive as they require a tedious experimental procedure and a sophisticated experimental device and performance. To overcome these defects, it is truly necessary to establish a real-time, on-site, and rapid detection method for human brucellosis. Here, a lateral flow immunoassay (LFIA) with a rapid, sensitive, and alternative diagnostic procedure for human brucellosis with a high degree of accuracy was developed based on blue silica nanoparticles (SiNPs), Staphylococcal protein A (SPA), and surface Lipopolysaccharide of Brucella spp. (LPS), which can be applied for rapid and feasible detection of human brucellosis. To our knowledge, this is the first report that uses blue SiNPs as a signal probe of LFIA for the rapid diagnosis of human brucellosis. The precursor of blue SiNPs@SPA such as colorless SiNPs and blue SiNPs was synthesized at first and then coupled with SPA onto the surface of blue SiNPs by covalent bond to prepare blue SiNPs@SPA as a capture signal to catch the antibody in the brucellosis-positive serum. When SPA was combined with the antibodies in the brucellosis-positive serum, it was captured by LPS on the test line, forming an antigen-antibody sandwich structure, resulting in the T line turning blue. Finally, the results showed that it is acceptable to use blue SiNPs as visible labels of LFIA, and standard brucellosis serum (containing Brucella spp. antibody at 1,000 IU/ml) could be detected at a dilution of 10-5 and the detection limit of this method was 0.01 IU/ml. Moreover, it also demonstrated good specificity and accuracy for the detection of real human serum samples. Above all, the blue SiNPs-based LFIA that we developed provides a rapid, highly accurate, and inexpensive on-site diagnosis of human brucellosis, and shows great promise in clinical diagnostics for other diseases.

Dataset Information

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of Salmonella typhimurium via a lateral flow immunoassay.

Publications

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of <i>Salmonella typhimurium via</i> a lateral flow immunoassay.

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