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A sensitive BODIPY-based fluorescent probe for detecting endogenous hydroxyl radicals in living cells.

ABSTRACT: The hydroxyl radical (˙OH) has been suggested to play very vital roles in many physiological and pathological processes. However, selective detection of ˙OH is highly challenging owing to its extremely high reactivity and short lifetime. Herein, we designed and synthesized a sensitive "turn on" fluorescent probe for detecting endogenous ˙OH based on a BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) platform. The probe had shown good properties including high sensitively, ideal selectively and low cytotoxicity, and was successfully employed to image endogenous hydroxyl radical in living cells.

SUBMITTER: Qu X

PROVIDER: S-EPMC9055842 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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A sensitive BODIPY-based fluorescent probe for detecting endogenous hydroxyl radicals in living cells.

Qu Xingyu X Bian Yongjun Y Chen Yongqiang Y Wei Xiaoqing X

RSC advances 20200804 48

The hydroxyl radical (˙OH) has been suggested to play very vital roles in many physiological and pathological processes. However, selective detection of ˙OH is highly challenging owing to its extremely high reactivity and short lifetime. Herein, we designed and synthesized a sensitive "turn on" fluorescent probe for detecting endogenous ˙OH based on a BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) platform. The probe had shown good properties including high sensitively, ...[more]

PMID: 35520039

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