Cas9-mediated gene editing in the black-legged tick, Ixodes scapularis, by embryo injection and ReMOT Control.
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ABSTRACT:
SUBMITTER: Sharma A
PROVIDER: S-EPMC9076890 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Cas9-mediated gene editing in the black-legged tick, <i>Ixodes scapularis</i>, by embryo injection and ReMOT Control.
Sharma Arvind A Pham Michael N MN Reyes Jeremiah B JB Chana Randeep R Yim Won C WC Heu Chan C CC Kim Donghun D Chaverra-Rodriguez Duverney D Rasgon Jason L JL Harrell Robert A RA Nuss Andrew B AB Gulia-Nuss Monika M
iScience 20220215 3
Despite their capacity to acquire and pass on an array of debilitating pathogens, research on ticks has lagged behind other arthropod vectors, such as mosquitoes, largely because of challenges in applying available genetic and molecular tools. CRISPR-Cas9 is transforming non-model organism research; however, successful gene editing has not yet been reported in ticks. Technical challenges for injecting tick embryos to attempt gene editing have further slowed research progress. Currently, no embry ...[more]
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