Single-cell profiling of transcriptomic changes during in vitro maturation of human oocytes.

ABSTRACT:

Purpose

In vitro maturation (IVM) of human oocytes offers an invaluable opportunity for infertility treatment. However, in vitro matured oocytes often show lower developmental abilities than their in vivo counterparts, and molecular mechanisms underlying successful maturation remain unclear. In this study, we investigated gene expression profiles of in vitro matured oocytes at the single-cell level to gain mechanistic insight into IVM of human oocytes.

Methods

Human oocytes were retrieved by follicular puncture and in vitro matured. In total, 19 oocytes from 11 patients were collected and subjected to single-cell RNA-seq analyses.

Results

Global gene expression profiles were similar among oocytes at the same maturation stage, while a

SUBMITTER: Takeuchi H 

PROVIDER: S-EPMC9084694 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature