Green and efficient biosynthesis of indigo from indole by engineered myoglobins.

ABSTRACT: With the demand nowadays for blue dyes, it is of practical importance to develop a green and efficient biocatalyst for the production of indigo. The design of artificial enzymes has been shown to be attractive in recent years. In a previous study, we engineered a single mutant of sperm whale myoglobin, F43Y Mb, with a novel Tyr-heme cross-link. In this study, we found that it can efficiently catalyze the oxidation of indole to indigo, with a yield as high as 54% compared to the highest yield (∼20%) reported to date in the literature. By further modifying the heme active site, we engineered a double mutant of F43Y/H64D Mb, which exhibited the highest catalytic efficiency (198 M^-1 s^-1) among the artificial enzymes designed in Mb. Moreover, both F43Y Mb and F43Y/H64D Mb

SUBMITTER: Liu C 

PROVIDER: S-EPMC9086478 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature