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Upcycling agro-industrial blueberry waste into platform chemicals and structured materials for application in marine environments.

ABSTRACT: Blueberry pruning waste (BPw), sourced as residues from agroforestry operations in Chile, was used to produce added-value products, including platform chemicals and materials. BPw fractionation was implemented using biobased solvents (γ-valerolactone, GVL) and pyrolysis (500 °C), yielding solid fractions that are rich in phenols and antioxidants. The liquid fraction was found to be enriched in sugars, acids, and amides. Alongside, filaments and 3D-printed meshes were produced via wet spinning and Direct-Ink-Writing (DIW), respectively. For the latter purpose, BPw was dissolved in an ionic liquid, 1-ethyl-3-methylimidazolium acetate ([emim][OAc]), and regenerated into lignocellulose filaments with highly aligned nanofibrils (wide-angle X-ray scattering) that simultaneously showed extensibility (wet strain as high as 39%). BPw-derived lignocellulose filaments showed a tenacity (up to 2.3 cN dtex^-1) that is comparable to that of rayon fibers and showed low light reflectance (R _ES factor <3%). Meanwhile, DIW of the respective gels led to meshes with up to 60% wet stretchability. The LCF and meshes were demonstrated to have reliable performance in marine environments. As a demonstration, we show the prospects of replacing plastic cords and other materials used to restore coral reefs on the coast of Mexico.

SUBMITTER: Reyes G

PROVIDER: S-EPMC9086861 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Upcycling agro-industrial blueberry waste into platform chemicals and structured materials for application in marine environments.

Reyes Guillermo G Pacheco Claudia M CM Isaza-Ferro Estefania E González Amaidy A Pasquier Eva E Alejandro-Martín Serguei S Arteaga-Peréz Luis E LE Carrillo Romina R RR Carrillo-Varela Isabel I Mendonça Regis Teixeira RT Flanigan Colleen C Rojas Orlando J OJ

Green chemistry : an international journal and green chemistry resource : GC 20220407 9

Blueberry pruning waste (BPw), sourced as residues from agroforestry operations in Chile, was used to produce added-value products, including platform chemicals and materials. BPw fractionation was implemented using biobased solvents (γ-valerolactone, GVL) and pyrolysis (500 °C), yielding solid fractions that are rich in phenols and antioxidants. The liquid fraction was found to be enriched in sugars, acids, and amides. Alongside, filaments and 3D-printed meshes were produced <i>via</i> wet spin ...[more]

PMID: 35694220

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Project description:BackgroundAshbya gossypii is a filamentous fungus widely utilized for industrial riboflavin production and has a great potential as a microbial chassis for synthesizing other valuable metabolites such as folates, biolipids, and limonene. Engineered strains of A. gossypii can effectively use various waste streams, including xylose-rich feedstocks. Notably, A. gossypii has been identified as a proficient biocatalyst for producing limonene from xylose-rich sources. This study aims to investigate the capability of engineered A. gossypii strains to produce various plant monoterpenes using agro-industrial waste as carbon sources.ResultsWe overexpressed heterologous terpene synthases to produce acyclic, monocyclic, and bicyclic monoterpenes in two genetic backgrounds of A. gossypii. These backgrounds included an NPP synthase orthogonal pathway and a mutant erg20F95W allele with reduced FPP synthase activity. Our findings demonstrate that A. gossypii can synthesize linalool, limonene, pinene, and sabinene, with terpene synthases showing differential substrate selectivity for NPP or GPP precursors. Additionally, co-overexpression of endogenous HMG1 and ERG12 with heterologous NPP synthase and terpene synthases significantly increased sabinene yields from xylose-containing media. Using mixed formulations of corn-cob lignocellulosic hydrolysates and either sugarcane or beet molasses, we achieved limonene and sabinene productions of 383 mg/L and 684.5 mg/L, respectively, the latter representing a significant improvement compared to other organisms in flask culture mode.ConclusionsEngineered A. gossypii strains serve as a suitable platform for assessing plant terpene synthase functionality and substrate selectivity in vivo, which are crucial to understand monoterpene bioproduction. The NPP synthase pathway markedly enhances limonene and sabinene production in A. gossypii, achieving levels comparable to those of other industrial microbial producers. Furthermore, these engineered strains offer a novel approach for producing monoterpenes through the valorization of agro-industrial wastes.

Dataset Information

Upcycling agro-industrial blueberry waste into platform chemicals and structured materials for application in marine environments.

Publications

Upcycling agro-industrial blueberry waste into platform chemicals and structured materials for application in marine environments.

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