FasL microgels induce immune acceptance of islet allografts in nonhuman primates.

ABSTRACT: Islet transplantation to treat insulin-dependent diabetes is greatly limited by the need for maintenance immunosuppression. We report a strategy through which cotransplantation of allogeneic islets and streptavidin (SA)-FasL-presenting microgels to the omentum under transient rapamycin monotherapy resulted in robust glycemic control, sustained C-peptide levels, and graft survival in diabetic nonhuman primates for >6 months. Surgical extraction of the graft resulted in prompt hyperglycemia. In contrast, animals receiving microgels without SA-FasL under the same rapamycin regimen rejected islet grafts acutely. Graft survival was associated with increased number of FoxP3⁺ cells in the graft site with no significant changes in T cell systemic frequencies or responses to donor and th

SUBMITTER: Lei J 

PROVIDER: S-EPMC9106299 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature