Application of Parallel Reaction Monitoring in ¹⁵N Labeled Samples for Quantification.

ABSTRACT: Accurate relative quantification is critical in proteomic studies. The incorporation of stable isotope ¹⁵N to plant-expressed proteins in vivo is a powerful tool for accurate quantification with a major advantage of reducing preparative and analytical variabilities. However, ¹⁵N labeling quantification has several challenges. Less identifications are often observed in the heavy-labeled samples because of incomplete labeling, resulting in missing values in reciprocal labeling experiments. Inaccurate quantification can happen when there is contamination from co-eluting peptides or chemical noise in the MS¹ survey scan. These drawbacks in quantification can be more pronounced in less abundant but biologically interesting proteins, which often have very

SUBMITTER: Reyes AV 

PROVIDER: S-EPMC9111532 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature