Transcriptional landscape of human microglia implicates age, sex, and APOE-related immunometabolic pathway perturbations.

ABSTRACT: Microglia have fundamental roles in health and disease; however, effects of age, sex, and genetic factors on human microglia have not been fully explored. We applied bulk and single-cell approaches to comprehensively characterize human microglia transcriptomes and their associations with age, sex, and APOE. We identified a novel microglial signature, characterized its expression in bulk tissue and single-cell microglia transcriptomes. We discovered microglial co-expression network modules associated with age, sex, and APOE-ε4 that are enriched for lipid and carbohydrate metabolism genes. Integrated analyses of modules with single-cell transcriptomes revealed significant overlap between age-associated module genes and both pro-inflammatory and disease-associated microglial clusters. These m

SUBMITTER: Patel T 

PROVIDER: S-EPMC9124307 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature